CUR Series Torrent Bioreactor

Low-shear, non-bubbling oxygen transfer from 2.5 L process development to 2,000 L production.

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Non-bubbling, low-shear oxygen transfer for sensitive suspension cell processes

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Scalable product family covering process development, pilot and production

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Online pH, DO, temperature, rotation speed and configurable process monitoring

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Supports batch, fed-batch and perfusion culture processes

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Suitable for HEK293, CHO, T cells, NK cells, iPSC and HS cells

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Description

Developed independently by JYSS BIO, the CUR Series Torrent Bioreactor is a single-use bioreactor that uses a non-bubbling interfacial oxygen-transfer mechanism for mixing. While meeting large-volume production requirements, this advanced mass-transfer method helps avoid cell damage caused by bubble surface tension and the high shear generated by impellers in conventional bioreactors.

Within the normal operating speed range, the mean shear rate inside the reactor can be lower than 20 s^-1, enabling high-density, high-viability culture of shear-sensitive cells. The series provides working volumes from 0.5 L to 2000 L for cell therapy, gene therapy, monoclonal antibody and vaccine applications.

Operating Principle

Stable wave motion and interfacial oxygen transfer across scales

The vessel moves in an overall planar circular motion, driving the liquid to form waves that wash the side walls and transfer oxygen into the liquid phase. This enables efficient oxygen dissolution in an extremely low-shear environment. The resulting stable and gentle flow field supports the growth and proliferation of fragile cells.

Features and Advantages

Non-bubbling interfacial oxygen transfer with high oxygen-transfer efficiency
No impeller or bottom sparger; no need for defoaming agents
Low shear; no need to add anti-shear factors
Inverted truncated-conical vessel with a large gas-liquid interface
Constant Froude number (Fr) scale-up for process reproducibility
High-density and high-viability cell culture
Smart control system with a simple operating interface
Online pH, DO, temperature and rotation-speed monitoring
Real-time data acquisition, reports and trend curves
Remote parameter and process monitoring
Electronic records and signatures in accordance with FDA 21 CFR Part 11
100% consumables integrity testing
Customizable tubing, accessories and film materials

Applications and Cell Culture

Suitable for suspension culture of HEK293, CHO, T cells, NK cells, iPSC and HS cells. Supported processes include batch, fed-batch and perfusion culture.

CHO Cell Perfusion Culture

During CHO cell perfusion culture in a CUR50 reactor, cell viability remained above 98%. The product literature reports a final protein expression level approximately 20% higher than with the compared culture methods.

HEK293T Cell Culture

For lentivirus production, cell density increased from 1.0×10⁶ cells/mL to 6.0×10⁶ cells/mL within three days, while viability remained above 90%. Viability stayed at 90% after transfection.

Monoclonal Antibody Culture and Scale-Up

CUR5L, CUR50L and CUR500L showed similar process performance, stability and scale-up consistency. Under the reported comparison conditions, peak cell density was at least 50% higher and monoclonal antibody yield was 40% higher than in the compared JYSS BIO agitator tanks using the same process.

System Components and Control

The system comprises a control tower and tank assembly. The tank consists of an oscillator and culture vessel. Outer surfaces use brushed 304 stainless-steel plates in line with GMP requirements, and electrical parts are CE certified.

Control Tower

Remote monitoring; real-time acquisition of pH, DO, temperature and speed; report and trend generation; Ethernet and USB interfaces; optional weighing, jacket temperature-control and perfusion modules.

Dissolved Oxygen Mixing System

Planar eccentric motion and a single-axis drive promote thorough gas-liquid mixing with low shear and high oxygen-transfer efficiency.

Inverted Truncated-Conical Vessel

The cylindrical-wall and conical structure creates a relatively large gas-liquid interface, supporting oxygen transfer and CO₂ removal.

Online Measurement

The pH module can link acid and alkali pumps with CO₂. The DO module can link N₂, O₂ and air valves for closed-loop control and gas-flow recording. Manual HMI control is also available.

Temperature and Pumping

A heating film provides continuous heating. An optional jacket module supports heating and cooling. Bidirectional peristaltic pumps can be used for medium addition, harvest, acid and alkali addition, and perfusion.

Technical Specifications

Specification	2.5 L	5 L	20 L	50 L	300 L	500 L	1200 L	2000 L
Overall dimensions (L×W×H), mm	678×441.5×550	1016×628×1435	1220×769×1437	1505×1115×1677	2196×1788×1990	2425×2045×2410	2618×2165×2658	Reactor: 2890×2890×3142.7 Control cabinet: 781.2×798.6×1577
Working volume (L)	0.5-2.5	2-5	6-20	15-50	50-300	120-500	150-1200	180-2000
Weight (kg)	66	155	289	595	1025	2072	2491	Reactor: 7784 Control cabinet: 147
Power (kW)	0.6	0.6	0.6	1	4	6.5	7.5	4
Optimal speed (rpm)	45-70	45-70	45-70	40-60	25-30	25-30	20-30	20-25
Speed range (rpm)	20-90	20-75	20-75	30-60	20-35	20-35	20-30	20-25
Speed accuracy	±1 rpm		±0.5 rpm
pH control	Digital smart electrode; range 0-12; accuracy ±0.02
DO control	Optical smart electrode; range 0-100% air saturation; accuracy ±5%
Temperature control	Two-point mode; room temperature to 42°C; accuracy ±0.2°C							Jacket control; 5-42°C; accuracy ±0.2°C
Conductivity control	Accuracy 0.1 mS/cm; range 0.02-500 mS/cm
Weighing display	Range defined as required. At ≤100 kg, precision error is 0.2 kg; at >100 kg, precision error is ±0.2%.

Frequently Asked Questions

What working volumes are available?

The CUR Series covers working volumes from 0.5 L to 2000 L.

Which cell types can be cultured?

It is suitable for HEK293, CHO, T cells, NK cells, iPSC and HS cells, including shear-sensitive cells.

Which processes are supported?

Batch, fed-batch and perfusion processes are supported.

How does the reactor reduce shear stress?

Planar circular vessel motion creates a stable, gentle flow field without an impeller or bottom sparger. Mean shear rate can be lower than 20 s^-1.

Is a defoaming agent required?

The non-bubbling design does not require a defoaming agent under the stated operating principle.

Can pH, DO and temperature be controlled?

Yes. Available online control and monitoring functions depend on the selected configuration.

Is remote monitoring available?

Yes. Remote monitoring and Ethernet/USB communication interfaces are supported.

Does the software support FDA 21 CFR Part 11?

Electronic records and electronic signatures are available. Confirm the required software configuration when ordering.

Can the system be customized?

Tubing, accessories and film materials can be customized. Optional modules include weighing, jacket temperature control and perfusion control.

Downloads

CUR Series Torrent Bioreactor Brochure

Product overview, specifications and application data (PDF)

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